马靓,茅国新

• 1:南通大学附属医院肿瘤化疗科

摘要(Abstract)：

目的检测非小细胞肺癌(non-small cell lung cancer,NSCLC)患者外周血中肺特异性X蛋白(LunxmRNA)、黏蛋白基因1(Muc1 mRNA)及CEA,探讨其对NSCLC微转移的诊断价值及临床意义。方法 72例NSCLC癌患者、10例肺良性疾病患者及12例健康志愿者,以逆转录-聚合酶链反应(reserve transcriptase-polymerasechain reaction,RT-PCR)方法,对其外周血进行Lunx mRNA和Muc1 mRNA的检测;以免疫化学发光测定法,检测其血清的CEA水平。结果 Lunx mRNA和Muc1 mRNA在NSCLC患者外周血中的表达率与肺良性病变组和正常对照组比较差异有统计学意义(P<0.01);其表达与NSCLC组织分化程度和临床分期相关(P<0.05),Muc1 mRNA的表达还与组织学类型有关(P<0.05)。以多种指标多种方法联合检测阳性率为最高。结论 Lunx mRNA和Muc1 mRNA是检测NSCLC微转移的良好指标。两者联合CEA检测可以提高肺癌检出率。

关键词(KeyWords)： 肺肿瘤;肿瘤转移;肺特异性X蛋白;黏蛋白基因1;癌胚抗原;逆转录聚合酶链反应

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 马靓,茅国新

DOI: 10.13267/j.cnki.syzlzz.2011.05.021

参考文献(References)：

• [1]Wu J,Ohta Y,Minato He,t al.Nodal occult metastasis inpatientswith peripheral lung adenocarcinoma of 2.0 cm orless in diameter[J].Ann Thorac Surg,2001,71(6):1772-1778.
• [2]Iwao K,Watanabe T,Fuiiwara Y,et al.Isolation of anovel human lung-specific gene,LUNX,a potentialmolecular marker for detection ofmicrometastasis in non-small cell lung cancer[J].Int J Cancer,2001,91(4):433-437.
• [3]Tezel C,Ersev AA,Kiral H,et al.The impact ofimmunohisto-chemical detection of positive lymph nodesin early stage lung cancer[J].Thorac Cardiovasc Surg,2006,54(2):124-127.
• [4]Sheu CC,Yu YP,Tsai JR,et al.Development of amembrane array-based multimarker assay for detection ofcirculating cancer cells in patients with non small celllung cancer[J].Int J Cancer,2006,119(6):1419-1426.
• [5]Nosotti M,Falleni M,Palleschi A,et al.Quantitative real-time polymerase chain reaction detection of lymph nodelung cancer micrometastasis using carcinoembryonicantigen marker[J].Chest,2005,128(3):1539-1544.
• [6]Wallace MB.Micrometastasis,molecular markers,andtheir future role with EUS-guided FNA[J].GastrointestEndosc2,0096,9(2):152-154.
• [7]Cheng M,Chen Y,Yu X,et al.Diagnostic utility of LunxmRNA in peripheral blood and pleural fluid in patientswith primary non-small cell lung cancer[J].BMCCancer,20088,:156.
• [8]王潍博,崔言刚,姚舒洋.肺特异性X蛋白角蛋白19及癌胚抗原基因在非小细胞肺癌淋巴结微转移中的比较[J].中华肿瘤杂志,2008,30(2):121-124.
• [9]Michael M,Loretta H,Gerard S,et al.Lunx is a superiormolecular marker for detection of non-small lung cellcancer in peripheral blood[J].J Mol Diagn,2003,5(4):237-242.
• [10]Coello MC,Luketich JD,Litle VR,et al.Prognosticsignificance of micrometastasis in non-small cell lungcancer[J].Clin Lung Cancer,2004,5(4):214-225.
• [11]Li SH,Wang Z,Liu XY,et al.Gene diagnosis andprognostic significance of lymph node micrometastasisafter complete resection of histologically node-negativenon-small cell lung cancer[J].World J Surg,20083,2(8):1651-1656.
• [12]Yamashita J,Matsuo A,Kurusu Y,et al.Preoperativeevidence of circulating tumor cells by means of reversetranscriptase-polymerase chain reaction forcarcinoembryonic antigen messenger RNA is anindependent predictor of survival in non-small cell lungcancer:a prospective study[J].Thorac CardiovascSurg2,0021,24(2):299-305.