孙成晖,曾奇,孙钦文

• 1:阳春市人民医院肿瘤科
• 2:中山大学附属第五医院肿瘤中心

摘要(Abstract)：

目的探讨晚期胃癌患者血浆RUNT相关转录因子3(human RUNT-related transcription factor 3,RUNX3)和p16基因甲基化与临床病理特征和氟尿嘧啶化疗敏感性的关系。方法收集行一线氟尿嘧啶联合化疗的晚期胃癌患者67例和体检健康42例。应用甲基化特异性PCR法检测血浆中RUNX3和p16基因甲基化,分析基因甲基化和临床病理特征、化疗疗效的关系。结果化疗前胃癌患者p16和RUNX3的甲基化频率均高于对照组[61.2%(41/67) vs 0.0%(0/42), 70.1%(47/67)vs 11.9%(5/42);均P<0.01]。化疗后胃癌患者p16和RUNX3的甲基化频率分别降低至47.8%(32/67;χ~2=5.05,P=0.025)和47.8%(38/67;χ~2=2.61,P=0.106)。以胃为主要肿瘤部位的患者血浆标本中p16和RUNX3的甲基化频率均高于以胃食管交界处为主要肿瘤部位的患者(均P<0.05)。p16和RUNX3甲基化阳性患者对氟尿嘧啶化疗的应答率均低于甲基化阴性患者(17.1%vs 42.3%, P=0.023; 19.1%vs45.0%, P=0.029)。结论 p16和RUNX3的甲基化在胃癌患者血浆中较为常见且特异性强,二者与患者对氟尿嘧啶化疗的反应密切相关。

关键词(KeyWords)： 胃肿瘤/病理学;胃肿瘤/血液;胃肿瘤/药物疗法;基因,p16;基因表达;甲基化;转录因子;氟尿嘧啶/治疗应用;药物疗法,联合;治疗结果

Abstract:

Keywords:

基金项目(Foundation): 阳江市卫计局科技计划项目(2015040)

作者(Author): 孙成晖,曾奇,孙钦文

DOI: 10.13267/j.cnki.syzlzz.2020.02.010

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