刘诗权,钟月圆,黄杰安,覃蒙斌,唐国都,姜海行

• 1:广西医科大学第一附属医院消化内科

摘要(Abstract)：

目的研究siRNA下调鞘氨醇激酶-1(Sphk1)的表达对结肠癌LOVO细胞增殖、凋亡、侵袭以及ERK和p38通路的影响。方法采用siRNA抑制Sphk1的表达;采用MTT法测定细胞的增殖,流式细胞术分析细胞的凋亡,Transwell小室检测细胞侵袭力的变化,Western杂交检测蛋白的表达;采用RT-PCR法检测细胞Sphk1 mRNA表达,ELISA法检测细胞基质金属蛋白酶(MMP)-2、MMP-9和尿激酶纤维蛋白溶酶原激活剂(uPA)的分泌。结果 Sphk1 siRNA显著抑制Sphk1 mRNA和蛋白表达,并可诱导细胞的凋亡,抑制细胞的增殖和侵袭。抑制Sphk1的表达可降低ERK和磷酸化ERK蛋白的表达,并促进p38和磷酸化p38蛋白的表达,同时可抑制细胞MMP-2、MMP-9和uPA的分泌。结论抑制Sphk1可能是通过抑制ERK并激活p38 MAPK通路,下调MMP-2、MMP-9和uPA的分泌,从而抑制结肠癌细胞的增殖和侵袭并促进细胞的凋亡。

关键词(KeyWords)： 结肠肿瘤;鞘氨醇激酶-1;细胞凋亡;肿瘤侵润;肿瘤细胞,培养的

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(30760275);; 广西科技厅留学回国基金项目(桂科回0832008);; 广西卫生厅基金项目(Z2008107)

作者(Author): 刘诗权,钟月圆,黄杰安,覃蒙斌,唐国都,姜海行

DOI: 10.13267/j.cnki.syzlzz.2011.05.019

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